Cloning and characterization of an aromatic amino acid and leucine permease of Penicillium chrysogenum.

PcMtr, an aromatic and neutral aliphatic amino acid permease of Penicillium chrysogenum.

The gene encoding an aromatic and neutral aliphatic amino acid permease of Penicillium chrysogenum was cloned, functionally expressed and characterized in Saccharomyces cerevisiae M4276. The permease, designated PcMtr, is structurally and functionally homologous to Mtr of Neurospora crassa, and unrelated to the Amino Acid Permease (AAP) family which includes most amino acid permeases in fungi. ...

Uptake of the -Lactam Precursor -Aminoadipic Acid in Penicillium chrysogenum Is Mediated by the Acidic and the General Amino Acid Permease

External addition of the -lactam precursor -aminoadipic acid to the filamentous fungus Penicillium chrysogenum leads to an increased intracellular -aminoadipic acid concentration and an increase in penicillin production. The exact route for -aminoadipic acid uptake is not known, although the general amino acid and acidic amino acid permeases have been implicated in this process. Their correspon...

Basic amino acid transport in plasma membrane vesicles of Penicillium chrysogenum.

The characteristics of the basic amino acid permease (system VI) of the filamentous fungus Penicillium chrysogenum were studied in plasma membranes fused with liposomes containing the beef heart mitochondrial cytochrome c oxidase. In the presence of reduced cytochrome c, the hybrid membranes accumulated the basic amino acids arginine and lysine. Inhibition studies with analogs revealed a narrow...

University of Groningen Uptake of the β-Lactam Precursor α-Aminoadipic Acid in Penicillium chrysogenum Is Mediated by the Acidic and the General Amino Acid Permease

Sulfate transport in Penicillium chrysogenum: cloning and characterization of the sutA and sutB genes.

In industrial fermentations, Penicillium chrysogenum uses sulfate as the source of sulfur for the biosynthesis of penicillin. By a PCR-based approach, two genes, sutA and sutB, whose encoded products belong to the SulP superfamily of sulfate permeases were isolated. Transformation of a sulfate uptake-negative sB3 mutant of Aspergillus nidulans with the sutB gene completely restored sulfate upta...